Operating Procedures for Imaging of Animals on mri scanners also used for Human Research Studies
Standard Operating Procedures for Imaging Animals on
MRI Scanners used for Human Research Studies
1. General Overview
The main principle to safely operating a dual-subject use facility is to ensure physical and temporal separation between human subjects and animals. Human subject and patient use must be the first priority. Animal use of the scanners can occur only when it will not conflict with human subjects being scanned.
Human subjects should never be able to directly see or hear, or otherwise identify that animals are nearby or were or are about to be used in the facility. This means that all supplies for animal work, and any wastes generated from animal work, must be removed before the facility is returned to human use. It also means that animals, especially non-human primates, must be anesthetized prior to transport into the facility.
The MR technicians will coordinate all movement of human subjects and animals in the MRRC magnet area.
2. Room air ventilation
Yale BS&P/Project Mgt confirms that the entire MR suite in TAC operates on 100% exhaust, meaning that none of the air is re-circulated back to either patient, animal, or other space inside the building.
Air clearance requirements:
3. Surface protection
All surfaces that animals come in contact with inside the facility must be covered with impermeable drapings or covering. Equipment and devices that will regularly be used for animals such as trays and carts should be dedicated to animal use only, labeled as such, and stored elsewhere.
Technologists must where gowns, masks, and gloves when dealing with sheep.Gowns and gloves are sufficient with dogs, pigs, rabbits, monkeys and rats.
All surfaces where animals had contact, even with impermeable coverings, must be disinfected after use with 10% bleach (1 part bleach + 9 parts water, made at least weekly). This is followed by a wipe down with water or 70% alcohol.
Responsibility for the disinfection of used surfaces lies with the MR technologist that ran the scan.
Animal excrement, blood or body fluids, hair, and any medical waste generated during the procedure or while in the dual use area must be appropriately packaged and removed from the facility after animal procedure is completed. This also applies to sharps containers, so that no potentially infectious materials from the animal(s) remain after they are removed. If any of these materials are spilled or otherwise contaminate areas inside the MR facility, the surface must be cleaned and decontaminated as for a human blood spill.
All the above waste should be transported out of the MRRC with the animal.
7. Medical Gases and Vacuum
Animals can use the same medical gas, scavenging, or vacuum systems used for humans as provided that different hoses and apparatus from the wall box out to the subjects are kept separate and dedicated to either human or non-human use. In all cases hoses must not be re-used and in the case of animals the hoses must be removed from the MRRC with the animal at the end of the experiment.
8. MR Equipment Operation
Operation of MR equipment must be limited to knowledgeable and authorized persons from the MRRC. All individuals involved must be trained in MR safety and understand other health and safety issues related to the facility.
9. Lab Animal Allergy Issues
Everyone involved in animal handling must review and complete the animal handlers health questionnaire, distributed through either YARC or the Employee Health Office. Since LAA's are fairly common, steps to minimize exposure to potential animal allergens must be considered in all aspects of animal work, including imaging and preparatory steps for imaging.
10. Non-Human Primates
NHPs pose unique and potentially more serious issues than many other lab animals, including physical assault injuries and bites, and infectious disease transmission. Individuals working directly with or around NHPs are encouraged to seek additional training and advice from YARC, especially regarding restraint systems, emergencies, and TB. For the MRRC, all non-human primates must arrive and leave under anesthesia.
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